his-72 wormbase gene report
NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.
4 Slice4D movies
pie-1%HIS-58%GFP+HIS-72%GFP_TDE1_slc.mov
pie-1%HIS-58%GFP+HIS-72%GFP_UDE1_slc.mov
pie-1%HIS-58%GFP+HIS-72%GFP_VDE1_slc.mov
HIS-72%GFP_E2_slc.mov
7 Stereo4D movies (4 embryos)
pie-1%HIS-58%GFP+HIS-72%GFP_TDE1_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%HIS-58%GFP+HIS-72%GFP_TDE1_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%HIS-58%GFP+HIS-72%GFP_UDE1_prx.mov (also viewable in ®© RedCyanStereo)
pie-1%HIS-58%GFP+HIS-72%GFP_UDE1_pry.mov (also viewable in ®© RedCyanStereo)
pie-1%HIS-58%GFP+HIS-72%GFP_VDE1_pryx.mov (also viewable in ®© RedCyanStereo)
HIS-72%GFP_E2_prx.mov (also viewable in ®© RedCyanStereo)
HIS-72%GFP_E2_pry.mov (also viewable in ®© RedCyanStereo)
GLOWorm = Gene/Lineage/Ontogeny Workspace for observation, reconstruction, and modeling.
3 comments:
Both markers are on integrated chromosomal arrays
Bead supported compression mounts ensure either ventral or dorsal view.
All three embryos present ventral view.
The his-72 is interesting, I wish I understood more about how it worked.
HISTONE%GFP-Embryo1 movies were created using SPIM imaging and deconvolution by Yicong Wu in Hari Shroff's group. Genotype of this embryo, provided by Zhirong Bao, is BV24 [ltIs44 [pie-1p-mCherry::PH(PLC1delta1) + unc-119(+)]; zuIs178 [(his-72 1kb::HIS-72::GFP); unc-119(+)] V]
But only gfp was imaged.
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