mab-21 wormbase gene report
NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.
3 Slice4D movies
mab-21%yfp_0bj_slc.mov
mab-21%yfp_1aa_slc.mov
mab-21%yfp_3bj_slc.mov
3 Stereo4D movies
mab-21%yfp_0bj_pryx.mov (also viewable in ®© RedCyanStereo)
mab-21%yfp_1aa_pryx.mov (also viewable in ®© RedCyanStereo)
mab-21%yfp_3bj_pryx.mov (also viewable in ®© RedCyanStereo)
GLOWorm = Gene/Lineage/Ontogeny Workspace for observation, reconstruction, and modeling.
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Movies made with hanging-embryo mounts (random DV/RL orientation for each embryo).
Integrated transgene array.
Expression begins ~230 min post fertilization in all 3 movies. Fluorescence very specific to fusion-fated hypodermal/epidermal precursor cells of hyp7+hyp6 syncytia, including both dorsal and some ventral cells. Remarkable match to expression pattern of the eff-1 gene, which encodes a cellular fusogen.
All three movies have the embryo misoriented with anterior to the right.
0Jbj = dorsolateral view
1jaa = dorsal view
3Jbj = lateral view
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