GLOWorm

View, analyze and annotate nematode anatomy and development.

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GLOWorm = Gene/Lineage/Ontogeny Workspace for observation, reconstruction, and modeling.

GLOWorm = Gene/Lineage/Ontogeny Workspace for observation, reconstruction, and modeling.

Immerse yourself in a multi-informatic experience of development and anatomy.

Example: WormAtlas, WormBase, EPiC and GLOWorm data integrated into a single interactive environment in GLOWormJ.

Explore anatomy and ultrastructure data from WormAtlas interactively through GLOWormJ.

Example: The nose region of the Slidable Worm annotated serial section project, with high-resolution images and web-linked annotations.

Example: The nerve ring of the N2U specimen, with high-resolution images, hand-drawn labels and web-linked annotations.

Example: The thermotaxis circuit of the JSH specimen, with high-resolution images and hand-drawn labels.

Explore gene-expression/lineage data from the EPIC project interactively through GLOWormJ.

Example: Histone::GFP (green) and pha-4::ceCHERRY (red) in a double-transgenic embryo, with cells auto-annotated and linked to web resources.

HMR-1::GFP translational fusion

hmr-1 wormbase gene report

NOTE: YouTube "thumbnails" are just an appetizer. Each genotype has several 4D movies that can be viewed interactively at full resolution.

10 Slice4D movies

DORSAL ASPECT
HMR-1%GFP_ADEA_slc.mov
HMR-1%GFP_ADEB_slc.mov
HMR-1%GFP_BDEA_slc.mov
HMR-1%GFP_BDEB_slc.mov
HMR-1%GFP_BDEC_slc.mov
HMR-1%GFP_CDEA_slc.mov
HMR-1%GFP_CDEB_slc.mov

VENTRAL ASPECT
HMR-1%GFP_AVEA_slc.mov
HMR-1%GFP_BVEA_slc.mov
HMR-1%GFP_BVEB_slc.mov

20 Stereo4D movies (10 embryos)

DORSAL ASPECT
HMR-1%GFP_ADEA_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_ADEA_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_ADEB_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_ADEB_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BDEA_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BDEA_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BDEB_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BDEB_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BDEC_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BDEC_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_CDEA_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_CDEA_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_CDEB_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_CDEB_pry.mov (also viewable in ®© RedCyanStereo)

VENTRAL ASPECT
HMR-1%GFP_AVEA_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_AVEA_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BVEA_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BVEA_pry.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BVEB_prx.mov (also viewable in ®© RedCyanStereo)
HMR-1%GFP_BVEB_pry.mov (also viewable in ®© RedCyanStereo)

2 comments:

wmohler said...: REFERENCE

A. Achilleos, A. M. Wehman and J. Nance (2010). PAR-3 mediates the initial clustering and apical localization of junction and polarity proteins during C. elegans intestinal epithelial cell polarization. Development 137: 1833-1842.; March 11, 2011 at 2:57 PM
Nora said...: Viewed JNHMR1ADE1prx.mov in stereo as well as with red cyan stereo in 3D. Visualized formation of intestinal region through tagged epithelial cells. Specifically viewable in time 20-40s.; March 30, 2011 at 7:46 PM

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For Mac (OS 10.5 or later) or Windows (XP or later): View/Analyze movies from the GLOWorm server

(Sorry, currently not Linux-compatible.)

GLOWormJ is a viewing/analysis program that allows you to watch, adjust contrast/color, synchronize, overlay, measure, annotate (etc.) movies from the GLOWorm collection, without waiting for very large movie files to download. You can choose multiple movies to watch in synchrony. You can capture clips of complex multi-movie comparisons for publication or to hardcopy your viewing experience.

Some sample scenes in GLOWormJ:

•4D DIC with cells hand-annotated and linked to web resources

•4D Histone::GFP with cells hand-annotated and linked to web resources

•4D Histone::GFP (green) overlayed with expression of pha-4::ceCHERRY (red) in a double-transgenic embryo. Cells are auto-annotated and linked to web resources.

•SPIM high-speed imaging of Histone::GFP (stereo4D): all of embryogenesis in 2-second time steps!

•Two-channel confocal slice4D

•Two-channel confocal stereo4D

•Side-by-side stereo (for uncrossed eyes)

•®© Red-Cyan stereo (for 3D glasses)

•Color-coded by depth in Z

•DeltaTime color-coded

•Two similar embryos superimposed in space and time

•Three embryos, each with a different fluorescence pattern, synchronized in space and time

Problems opening a second or third movie/scene in GLOWormJ?

Clicking a link to a movie or scene may not work on your computer if you already have an instance of GLOWormJ running. But all you need to do is drag the link from the webpage to the GLOWormJ toolbar, and the movie will open in the same copy of GLOWormJ you already have running.

You can also open any .mov or .avi file you have on your own computer by drag-dropping this same way.

Troubleshooting GLOWormJ on your system? You may need these one-time installations:

Java must be installed on your computer, and must be version 1.5 or later. Click this Java link to test whether your installation is up-to-date. If you are on a Mac, you must be running OS 10.5 or later for your Java to be up-to-date.

Using GLOWormJ also requires QuickTime on Mac and Windows machines. If asked by GLOWormJ to install QuickTime, please follow the posted link and run the QuickTime installer for your system. iTunes in not required to run GLOWormJ.

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